Saturday, February 15, 2020
DNA Fingerprinting Research Paper Example | Topics and Well Written Essays - 1250 words
DNA Fingerprinting - Research Paper Example The high rate of variation results because DNA fingerprinting relies on non-coding hyper-variable sequences to produce a unique pattern of bands for each individual. DNA profiling relies on the discovery of a broad range of restriction enzymes and their specificity. DNA typing has a wide range of applications from paternity testing, criminal investigations, and population studies to identification of tragedy victims. Other applications are in conservation biology and evolution studies. However, DNA typing presents its challenges especially concerning the amount of sample and accuracy of the process. Introduction DNA fingerprinting has caused a revolution in the world since its description in 1985. Deoxyribonucleic acid is present in all body cells. DNA consists of a sugar, four nucleotides, and a phosphate group. The nucleotides commonly called bases differ in the frequency of occurrence and the order in which they occur. The general DNA structure is similar in all individuals. Howev er, the order and frequency of bases brings a remarkable difference between individuals. DNA fingerprinting presents a profile of an individualââ¬â¢s DNA. The four bases namely adenine, cytosine, thymine, and guanine form unique sequences on the two DNA chromosomes. Studies reveal that there are sequences that encode for essential proteins that are necessary for all cell functions. Geneticists called these coding sequences exons. In addition, there are non-coding sequences, the introns. Studies have revealed that the coding sequences are present in every individual because they code for proteins that drive the life process. These sequences have great similarity in individuals and display limited variation. On the other hand, the non-coding sequences portray a high level of variation and form the basis of DNA profiling. Basis of Fingerprinting DNA profiling is currently the most powerful tool in individual identification. It utilizes the variation of the non-coding sequences to pr oduce unique profiles for each individual (Starr et al 247). The variation in these sequences is too high and this minimizes the probability of two individuals having identical profiles to virtually zero. Due to their high level of variability, geneticists call them hyper- variable regions. These regions consist of about ten to fifteen core sequences that may repeat themselves severally at different locations in the chromosome. The non-coding regions appear in between the coding regions. The frequency of repetition of these highly variable regions results to the differences among individuals. Studies indicate that only identical twins produce similar DNA profiles. The reliability on DNA profiles overrides the traditional fingerprints. The environment contributes greatly to the patterns of the fingers of an individual and the method presented its challenges. DNA fingerprinting presents a great potential in providing accurate profiles that can differentiate two individuals. Closely re lated individuals display a level of similarity in the profiles depending on the level of correlation. Procedure of Running a DNA Fingerprint DNA fingerprinting is laboratory technology involving several procedures. The discovery of restriction enzymes, which cleave DNA at specific recognition sites, formed the stepping-stone to DNA fingerprinting. The initial step in DNA typing is the isolation of DNA from the sample. Samples may be blood, cells, saliva, urine, hair follicles, bones, teeth, and hair fragments (Read 21). Geneticists recognize the existence of both nuclear DNA found in the cell nucleus and mitochondrial DNA in the mitochondrion. The amount of sample available determines the type of DNA isolated. In cases where small samples are available
Sunday, February 2, 2020
Online Inventory Essay Example | Topics and Well Written Essays - 500 words
Online Inventory - Essay Example As the discussion highlights his learning styles are visual, physical, social, aural, verbal, logical, and physical according to learning-styles-online.com and; sensitive, active, sequential, visual, solitary and referral according to Solomon and Felder. In terms of visual, the author pay attention to images to understand while, in verbal terms, use of explanation is useful to him. He scored highly on visual learning skills as compared to verbal. Images help him to remember what was taught in class and thus very effective on the author.This discussion stresses that there were similarities and differences in my study methods as portrayed by the two different websites. The similarities were that my learning styles are physical or active of which he do enjoy exercising and sports for instance football and basketball.à Another common aspect is that images help me study better and achieve my goals. On the other hand, the major differences is that his learning style is social, and solit ary. The differences might have resulted from the question structure which led to his different interpretation on the same. The authorà found out that he has to improve some certain aspects like verbal, social and logical learning styles. The hints given by the two websites provided a platform for him to improve and make himself better.à The author has to improve on his verbal skills because visual learning requires explanations.
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